The Water Houses, built by ACEA S.p.A., are modern facilities for the distribution of chilled natural and sparkling water, located in spaces accessible to citizens. The water supplied comes from the city's aqueducts and undergoes rigorous periodic checks by ACEA and the relevant local health authorities, guaranteeing quality and safety. Each point dispenses water at a flow rate of 180 l/h, allowing a 1-litre bottle to be filled in 20 seconds. In addition to the ongoing installation plan by ACEA S.p.A., new Water Houses will be placed at the following strategic locations during the Jubilee:

- Largo Pietro Tacchi Venturi;

- Piazza Giuseppe Gioacchino Belli;

- Piazza di Porta San Giovanni;

- Piazza di Santa Croce in Gerusalemme;

- Viale di San Paolo;

- Piazzale di San Lorenzo;

- Termini Station;

- Via della Stazione Tiburtina;

- Piazza Pia;

- Piazza del Risorgimento;

- Piazza di Santa Maria Maggiore;

- Piazza di Santa Maria Liberatrice;

- Piazza della Città Leonina;

- Viale Vaticano.